| dr hab. Dario Piano |
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Dr hab. Piano Dario pok. 0/131, tel. 225932534 e-mail: dario_piano@sggw.pl
Research interests Basic research : · Structural and functional studies of Photosystem II from higher plants; · Mechanisms of photoprotection and Non-Photochemical Quenching (NPQ) in higher plants; · Mechanisms of S-layer-mediated photoprotection and carotenoid-mediated photoprotection in radio-, thermo-, and halo-extremophile bacteria; · Structural and functional studies of bacterial S-layers; · Role of amino acids in rhizobia attraction during early stages of nodulation. Applied Research: · Development and implementation of biochemical techniques and methods for industrial production and applications of bacterial S-layers and bacterial carotenoids.
Education and scientific career: 2018-present - Group leader at the Warsaw University of Life Sciences, Department of Plant Physiology, Warsaw (Poland). Project: “Structural and functional characterization of SlpA the main S-layer protein of Deinococcus radiodurans”. Secondary projects: “Structural characterization of several Photosystem II subtypes isolated from Nicotiana tabacum”. 2017 - Italian National habilitation for positions of associated professor in Plant Physiology. 2015-2016 - Independent researcher and Junior professor position in Plant Physiology at the University of Cagliari (Department Life and Environmental Sciences ), Cagliari (Italy). Projects:“Three dimensional crystallization and X-ray diffraction analysis of Photosystem II crystals from several transplastomic strains of Nicotiana tabacum” and “Structural studies of the surface-layer protein complexes from the radio-resistant bacterium Deinococcus radiodurans”. 2013-2015 – Researcher position at the International Institute of Molecular and Cell Biology, Department of Structural Biology, Warsaw (Poland). Projects:“Structural and functional characterization of Photosystem II from Nicotiana tabacum”. 2010-2013 - Independent researcher and Junior professor position in Plant Physiology at the University of Cagliari (Department Life and Environmental Sciences ), Cagliari (Italy). Projects:“Three dimensional crystallization and X-ray diffraction analysis of Photosystem II crystals from several transplastomic strains of Nicotiana tabacum” and “Structural studies of the surface-layer protein complexes from the radio-resistant bacterium Deinococcus radiodurans”. 2009-2010 - Post-doc position at the European Synchrotron Radiation Facility (ESRF), Macromolecular Crystallography Group, Grenoble (France). Projects: “Purification and crystallization of the surface-layer protein complexes from the radio-resistant bacterium Deinococcus radiodurans” Secondary projects: “Three dimensional crystallization and X-Ray diffraction analysis of the E. coli ATP synthase” and “Three dimensional crystallization and X-ray diffraction analysis of Photosystem II crystals from several transplastomic strains of Nicotiana tabacum”. 2007-2009 - Post-doc position at the International Institute of Molecular and Cell Biology, Department of Structural Biology, Warsaw (Poland). Projects:“Three dimensional crystallization, X-ray analysis and oxygen evolution studies of Photosystem II from Nicotiana tabacum” and “Functional and structural analysis of bacterial membrane proteases”. 2004-2007 - PhD degree in Biology. Johann Wolfgang Goethe University, Institute of Molecular Biosciences Department of Plant Cell Biology, Frankfurt am Main (Germany) and Max Planck Institute for Biophysics, Department of Structural Biology, Frankfurt am Main (Germany). PhD thesis: “ Structural and functional characterization of Photosystem II from two His-tagged transplastomic strains of Nicotiana tabacum”. 2002-2003 - Master Degree in Natural Sciences. Master thesis: “Genetically modified organisms: techniques and related issues”; Department of Genetics, University of Cagliari (Italy). 1995-2001 - Master Degree in Biology. Master thesis: “Indolcatechol derivatives: new inhibitors of the HIV-1 integrase”; Department of Experimental Biology, University of Cagliari (Italy).
Domenica Farci, Stefano Francesco Farci, Francesca Esposito, Enzo Tramontano, Joanna Kirkpatrick, Dario Piano. On the S-layer of Thermus thermophilus and the assembling of its main protein SlpA. Biochim. Biophys. Acta – Biomembranes. 2018, 1860: 1554-1562. doi: 10.1016/j.bbamem.2018.05.010. Venkata Krishnan Ramaswamy, Francesco Di Palma, Attilio V. Vargiu, Angela Corona, Dario Piano, Paolo Ruggerone, Luca Zinzula, EnzoTramontano. Insights into the homo-oligomerization properties of N-terminal coiled-coil domain of Ebola virus VP35 protein. Virus Res. 2018, 247:61-70. doi: 10.1016/j.virusres.2018.02.003. Cinzia Sanna, Daniela Rigano, Angela Corona, Dario Piano, Carmen Formisano, Domenica Farci, Genni Franzini, Mauro Ballero, Giuseppina Chianese, Enzo Tramontano, Orazio Taglialatela-Scafati, Francesca Esposito. Dual HIV-1 reverse transcriptase and integrase inhibitors from Limonium morisianum Arrigoni, an endemic species of Sardinia (Italy). Nat Prod Res. 2018, 4:1-6. doi: 10.1080/14786419.2018.1434649. Domenica Farci, Chavdar Slavov, Dario Piano. Coexisting properties of thermostability and Ultraviolet radiation resistance in the main S-layer complex of Deinococcus radiodurans. Photochem Photobiol Sci. 2018, 17:81-88. doi: 10.1039/c7pp00240h. Domenica Farci, Francesca Esposito, Sabah El Alaoui, Dario Piano. S-layer proteins as a source of carotenoids: isolation of the protein cofactor deinoxanthin from its S-layer protein DR_2577. Food Res Intern, 2017, doi: 10.1016/j.foodres.2016.10.003. Francesca Esposito, Manuela Mandrone, Claudia Del Vecchio, Ilaria Carli, Simona Distinto, Angela Corona, Mariacaterina Lianza, Dario Piano, Massimo Tacchini, Elias Maccioni, Filippo Cottiglia, Elisa Saccon, Ferruccio Poli, Cristina Parolin, Enzo Tramontano. Multi-target activity of Hemidesmus indicus decoction against innovative HIV-1 drug targets and characterization of Lupeol mode of action. Pathog Dis. 2017, doi: 10.1093/femspd/ftx065. Gabriella Collu, Domenica Farci, Francesca Esposito, Francesca Pintus, Joanna Kirkpatrick, Dario Piano. New insights into the operative network of FaEO, an enone oxidoreductase from Fragaria x ananassa Duch. Plant Mol Biol, 2017, doi:10.1007/s11103-017-0597-5. Domenica Farci, Joanna Kirkpatrick, Dario Piano. A new procedure for a fast soft staining of BN-PAGEs on photosynthetic complexes. Electrophoresis, 2017, 38: 441-6. doi: 10.1002/elps.201600389. Francesca Esposito, Carli Ilaria, Del Vecchio Claudia, Xu Lijia, Angela Corona, Nicole Grandi, Dario Piano, Elias Maccioni, Simona Distinto, Parolin Cristina, Enzo Tramontano. Sennoside A, derived from the traditional chinese medicine plant Rheum L., is a new dual HIV-1 inhibitor effective on HIV-1 replication. Phytomedicine, 2016, 23: 1383-1391. Domenica Farci, Gabriella Collu, Joanna Kirkpatrick, Francesca Esposito, Dario Piano. RhVI1 is a membrane-anchored vacuolar invertase highly expressed in Rosa hybrida L. petals. J Exp Bot. 2016, 67:3303-12. doi: 10.1093/jxb/erw148. Domenica Farci, Chavdar Slavov, Enzo Tramontano, Dario Piano. The S-layer protein DR_2577 binds the carotenoid deinoxanthin and under desiccation conditions protect against UV-radiation in Deinococcus radiodurans. Frontiers in Microbiology, section of Extreme Microbiology, research topic Photobiology of Extremophiles, Front Microbiol. 2016, 7:155. doi: 10.3389/fmicb.2016.00155. Patrycja Haniewicz, Davide Floris, Domenica Farci, Joanna Kirkpatrick, M. Cecilia Loi, Claudia Büchel, Matthias Bochtler, Dario Piano. Isolation of plant Photosystem II complexes by fractional solubilisation. Frontiers in Plant Sciences, Plant Cell Biology journal, research topic Assembly of the Photosystem II Membrane-Protein Complex of Oxygenic Photosynthesis, Front Plant Sci. 2015, 6:1100. doi: 10.3389/fpls.2015.01100. Domenica Farci, Matthew W. Bowler, Francesca Esposito, Sean McSweeney, Enzo Tramontano, and Dario Piano. Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans. Front in Microbiol, 2015, 6:414. doi:10.3389/fmicb.2015.00414. Lijia Xu, Nicole Grandi, Claudia Del Vecchio, Daniela Mandas, Angela Corona, Dario Piano, Francesca Esposito, Cristina Parolin, Enzo Tramontano. From the traditional Chinese medicine plant Schisandra chinensis new scaffolds effective on HIV-1 reverse transcriptase resistant to non-nucleoside inhibitors. J. Microbiol 2015, 53(4):288-293. Domenica Farci, Matthew W. Bowler, Joanna Kirkpatrick, Sean McSweeney, EnzoTramontano and Dario Piano. New features of the cell wall of the radio-resistant bacterium Deinococcus radiodurans. Biochim. Biophys. Acta - Biomembranes. 2014, 1838: 1978-84. doi:10.1016/j.bbamem.2014.02.014. Patrycja Haniewicz, Daniele De sanctis, Claudia Büchel, Wolfgang P. Schröder, Maria Cecilia Loi, Thomas Kieselbach, Matthias Bochtler, Dario Piano. Isolation of monomeric photosystem II that retains the subunit PsbS. Photosynth Res 2013, 118(3): 199-207. Erika Pellegrini, Dario Piano, Matthew W. Bowler. Direct cryocooling of naked crystals: are cryoprotection agents always necessary? Acta Cryst. 2011, D67, 902–906. Dario Piano, Sabah El Alaoui, Henryk J. Korza, Renata Filipek, Izabela Sabala, Pratycja Haniewicz, Claudia Büchel, Daniele De Sanctis, Matthias Bochtler. Crystallization of the Photosystem II core complex and its chlorophyll binding subunit CP43 from a transplastomic strain of Nicotiana tabacum. Photosynth. Res. 2010, 106 (3):221-6. Luca Zinzula, Francesca Esposito, Elke Muhlberger, Martina Trunschke, Dominik Conrad, Dario Piano, Enzo Tramontano. Functional Characterisation of the full lenght Ebola Virus VP35 protein expressed in E. coli. Protein Expr. and Purif. 2009, 66 (1):113-9.
Recent intarnational conferences attended: Gordon Research Conference on Carotenoids, Apocarotenoids and Retinoids: from Nature to Bedside. 17-22 June 2018, Grand Summit Hotel at Sunday River, Newry, ME, US. European Society for Photobiology 2017 Congress, Pisa, Italy, 4-8 September 2017. Joint Congress of the French and Italian Photochemists and Photobiologists. September 19-22, 2016 Università Aldo Moro, Bari (IT). Gordon Research Conference. Carotenoid Biosynthesis, Functions, and Application to Human Health. May 22-27, 2016 Renaissance Tuscany il Ciocco , Pisa (IT). Gordon Research Conference. Photosynthesis From Evolution of Fundamental Mechanisms to Radical Re-Engineering. August 10-15, 2014 Mount Snow Resort West Dover (US). |
